Suspected Cholera Outbreak in Wardak Province, Afghanistan, 2022: a teaching case study of laboratory role in outbreaks investigation
Shoaib Naeemi, Khwaja Mir Islam Saeed, Mir Salamuddin Hakim
Corresponding author: Shoaib Naeemi, Eastern Mediterranean Public Health Network (EMPHNET) Afghanistan Country Office, Kabul City, Afghanistan 
Received: 25 Dec 2024 - Accepted: 25 Oct 2025 - Published: 17 Feb 2026
Domain: Infectious diseases epidemiology,Laboratory medicine,Public health
Keywords: Diarrhea, acute watery diarrhea, laboratory, infection prevention, outbreak, epidemiology, Afghanistan
Funding: This work received no specific grant from any funding agency in the public, commercial, or non-profit sectors.
This article is published as part of the supplement Teaching Case Studies in Field Epidemiology and Public Health for the Eastern Mediterranean Region, commissioned by Eastern Mediterranean Public Health Network (EMPHNET).
©Shoaib Naeemi et al. Pan African Medical Journal (ISSN: 1937-8688). This is an Open Access article distributed under the terms of the Creative Commons Attribution International 4.0 License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Cite this article: Shoaib Naeemi et al. Suspected Cholera Outbreak in Wardak Province, Afghanistan, 2022: a teaching case study of laboratory role in outbreaks investigation. Pan African Medical Journal. 2026;53(1):2. [doi: 10.11604/pamj.supp.2026.53.1.46341]
Available online at: https://www.panafrican-med-journal.com//content/series/53/1/2/full
Case study 
Suspected Cholera Outbreak in Wardak Province, Afghanistan, 2022: a teaching case study of laboratory role in outbreaks investigation
Suspected Cholera Outbreak in Wardak Province, Afghanistan, 2022: a teaching case study of laboratory role in outbreaks investigation
Shoaib Naeemi1,&, Khwaja Mir Islam Saeed2, Mir Salamuddin Hakim2
&Corresponding author
This teaching case study highlights the crucial role that laboratories play in the investigation and management of disease outbreaks. Designed specifically for participants in the intermediate tier of the Field Epidemiology Training Program (FETP), the case study aims to deepen the understanding of laboratory contributions to outbreak response efforts. By focusing on a real-life outbreak scenario, it provides practical, hands-on learning experiences that bridge theoretical knowledge with field applications. The case study is grounded in an actual outbreak of Acute Watery Diarrhea (AWD) that affected two villages in the Jalrez District of Wardak province in July 2022. The primary objective of this teaching case study is to educate FETP residents about the significance of laboratories in outbreak investigations. By exploring the AWD outbreak, participants will learn about various aspects of laboratory involvement, including specimen types, collection procedures, and proper management of collected samples.
Key learning points:
1. Laboratory's role in outbreak investigations: participants will gain insight into how laboratories play a critical role in confirming outbreaks, identifying pathogens, and guiding control measures. They will understand the importance of accurate and timely laboratory results in informing public health decisions.
2. Specimen types and collection: the case study delves into the different types of specimens used in outbreak investigations, with a focus on AWD. Participants will be challenged to discuss on which specimens are most appropriate for diagnosing specific diseases and the significance of sample timing in obtaining reliable results.
3. Specimen collection procedures: FETP residents will be guided through proper specimen collection techniques, emphasizing the importance of maintaining sample integrity to ensure accurate laboratory analysis. They will learn how to minimize contamination risks and ensure representative sampling.
4. Specimen management: this section highlights the significance of proper specimen management, including storage, transportation, and documentation.
5. Laboratory-field collaboration: the case study underscores the importance of effective communication and collaboration between field epidemiologists and laboratory personnel. Participants will learn how to facilitate seamless information exchange and coordination to ensure efficient outbreak response.
6. Real-life application: by examining the AWD outbreak in two villages of the Jalrez District, participants will gain practical insights into the challenges and complexities of real-world outbreak investigations. This context allows for a comprehensive understanding of the concepts discussed.
7. Public Health Decision-Making: through the lens of the AWD outbreak, FETP residents will explore how laboratory results influence public health decisions, from implementing control measures to resource allocation.
Time allotted: 1-2 Hours
Language: English
Groups: 3-5 residents per group
Materials required: pen, pencil, flip chart, calculator, marker, highlighter pen, and personal computer (if available)
- Download the case study student guide;
- Request the case study facilitator guide.
The authors declare no competing interests.
Afghanistan Field Epidemiology Training Program (AFETP) extend their gratitude to National Diseases Surveillance and Response (NDSR) department of Wardak Provincial Public Health Directorate, Central Public Health Laboratories of Afghanistan under the roof of Monitoring, Evaluation and Health Information System (M&EHIS) General Directorate of Ministry of Public Health for providing data and information of this teaching case study.
- Global Task Force for Cholera Control (GTFCC). GTFCC Cholera Outbreak Response Field Manual. 2019;1-2.
- WHO. Diarrhoeal disease. World Health Organization. Cited 2023 Aug 26.
- Fanous M, King KC. Cholera. [Updated 2023 May 23]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan-3. PMID: 29262189. PubMed
- Deen J, Mengel MA, Clemens JD. Epidemiology of cholera. Vaccine. 2020 Feb 29;38 Suppl 1:A31-A40. PubMed | Google Scholar
- World Health Organization (WHO). Infectious Disease Outbreaks in Afghanistan: Weekly Report, Week 01, 2023. Cited 2023 Aug 26.



