In vivo antiplasmodial activities of stem bark extracts of Avicennia marina in Plasmodium berghei-infected mice
Gitau Wilfred, Edinah Kwamboka Songoro, Jeremiah Waweru Gathirwa, Francis Kimani, Humphrey Njaanake Kariuki
Corresponding author: Gitau Wilfred, Department of Medical Microbiology, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
Received: 06 Dec 2022 - Accepted: 13 Jan 2023 - Published: 16 Feb 2023
Domain: Parasitology,Pharmacology,Medicinal chemistry
Keywords: Malaria, Avicenia marina, P. berghei, mice, suppression
©Gitau Wilfred et al. Pan African Medical Journal (ISSN: 1937-8688). This is an Open Access article distributed under the terms of the Creative Commons Attribution International 4.0 License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Cite this article: Gitau Wilfred et al. In vivo antiplasmodial activities of stem bark extracts of Avicennia marina in Plasmodium berghei-infected mice. Pan African Medical Journal. 2023;44:93. [doi: 10.11604/pamj.2023.44.93.38448]
Available online at: https://www.panafrican-med-journal.com/content/article/44/93/full
In vivo antiplasmodial activities of stem bark extracts of Avicennia marina in Plasmodium berghei-infected mice
In vivo antiplasmodial activities of stem bark extracts of Avicennia marina in Plasmodium berghei-infected mice
Gitau Wilfred1,&, Edinah Kwamboka Songoro1, Jeremiah Waweru Gathirwa2, Francis Kimani3, Humphrey Njaanake Kariuki4
Introduction: malaria remains the leading cause of morbidity and mortality in developing tropical and subtropical nations. Due to the emergence and spread of drug resistance to currently available drugs, there is a need for the search of novel, safe, and reasonably affordable anti-malarial medications. The objective of this study was to assess the in vivo anti-malarial effectiveness of Avicennia marina stem bark extracts in a mice model.
Methods: guidelines 425 of the Organization for Economic Cooperation and Development were used to determine the extracts' acute toxicity. Mice infected with chloroquine-sensitive Plasmodium berghei (ANKA strain) were tested for in vivo anti-plasmodial activity, and by giving oral doses of 100 mg/kg, 250 mg/kg, and 500 mg/kg body weight of extracts, the plant's suppressive, curative, and preventive effects were assessed.
Results: mice treated with dosages of up to 5000 mg/kg showed no evidence of acute toxicity or mortality. Consequently, it was determined that the acute lethal dosage of Avicennia marina extracts in swiss albino mice was greater than 5000 mg/kg. All doses of the extracts exhibited significant (p<0.05) dose-dependent suppression of P. berghei in the suppressive tests compared to the control group. At the highest dose (500 mg/kg), Methanolic crude extracts exerted the highest (93%) parasitemia suppression during the 4-day suppressive test. The extracts also displayed significant (p<0.001) prophylactic and curative activities at all doses compared to the control.
Conclusion: results from this study ascertained the safety and promising curative, prophylactic and suppressive anti-plasmodial capabilities of the stem bark extracts of Avicennia marina in mice model.
Malaria is an infectious, life-threatening disease caused by Plasmodium (Plasmodium vivax, Plasmodium malariae, Plasmodium ovale, and Plasmodium knowlesi) species which are transmitted through the bites of infected female Anopheles mosquitoes . The disease remains a major source of morbidity and mortality, especially in tropical developing countries like Kenya, where children under 5 and pregnant women are most at risk . Globally, nearly half the population is at risk of contracting malaria, with African regions bearing the highest burden . According to World Health Organization´s (WHO´s) 2019 report, an estimated 228 million cases of malaria infections were reported globally with 93% of those cases taking place in African region, followed by the WHO South-East Asia Region (3.4%) and the WHO Eastern Mediterranean Region (2.1%) . Of the estimated 405,000 malaria-related deaths globally, 67% were children under the age of five, with 94% of the fatalities occuring in the WHO African Region . With an estimated 3.5 million new clinical cases and 10,700 deaths each year, malaria is still a major public health concern in Kenya, accounting for 13% to 15% of outpatient visits .
Since the isolation of quinine in 1820s as the first effective anti-malarial drug, a number of other natural and synthetic compounds have been developed for treatment of the disease . For example, chloroquine, a synthetic compound of quinoline was introduced in the 1940s to treat all forms of malaria and the population-based prophylaxis with this drug was initiated in many areas in the 1960s . Despite widespread resistance to the drug throughout the tropics in the 1980s, chloroquine treatment for uncomplicated P. falciparum malaria is still utilized in some countries where the strains are sensitive . Currently, WHO Model List of Essential Medicines has listed 14 drugs for curative treatment, and 4 for prophylaxis against malaria, formulated either as single compounds or as combinations . WHO has recommended artemisinin, in combination with drugs such as amodiaquine, lumefantrine, mefloquine, and sulphadoxine-pyrimethamine as first-line treatment of uncomplicated falciparum malaria and parenteral artesunate for the treatment of severe malaria in endemic zones . In sub-Saharan African, pyrethroid insecticides are used in malaria prevention efforts, both for indoor residual spraying (IRS) and long-lasting insecticide nets (LLINs) . Other strategies used to lower the number of malaria cases, include entomologic surveillance, and intermittent preventative therapy for expectant mothers in malaria-endemic areas . However, the emergence of pyrethroid-resistant mosquitoes in Western Kenya, prompted the government to stop spraying insecticide against mosquitoes between 2013 and 2016 . Malaria parasites resistant to quinine and antifolate derivatives has been widely reported . The emergence and geographic expansion of artemisinin resistant P. falciparum in the Greater Mekong Subregion (GMS) poses a severe challenge in efforts to control and eradicate malaria globally . Studies conducted recently have established the rise of partial resistance to artemisinin in some African regions, notably in Eritrea, Rwanda, and Uganda [14,15]. Given Africa's extensive reliance on artemisinin-based combination therapies (ACTs) as the first-line treatment of uncomplicated malaria, the issue of artemisinin partial resistance and partner drug resistance must be closely monitored and addressed urgently through innovative development of novel anti-malarial drugs [8,16].
Globally, plants have extensively been used in traditional medicine to treat illnesses including malaria, fever, cholera, diarrhoea, pain, breast swelling, elephantiasis, inflammation, flu, dyslipidemia and hyperglycaemia particularly in endemic areas [17-21]. Artemisinin and quinine, the two main groups of the most effective antimalarial drugs, are derived from plants Artemisia annua and Cinchona sp., respectively . In an effort to find new natural antimalarial medicine in Kenya, several studies have been conducted on various plants which are traditionally used by the local communities to treat malaria [11,23,24].
The seashore ecosystem serves as a source of food, medicine, fuel, and building materials to local communities and one of the widely distributed plants along seashores is mangrove [21,25]. Mangroves have extensively been explored for their versatile medicinal uses which include the treatment of abscesses, ulcers, flu, rheumatism, smallpox, cancer, malaria, scabies, sores and boils [25-31].
Avicennia marina (Forsk.) Vierh, (gray mangrove) is one of the mangrove species which is widely distributed within a latitudinal range of 30°N to 38°S with a global distribution in intertidal sections of seabeds, estuaries, rivers, and streams [32,33]. Several studies evaluating the antiplasmodial activities of A. marina tree extracts have been conducted with results showing varying degrees of efficacy [8,34]. Previous research has also revealed the presence of phytochemicals such as saponin, alkaloids, and phenolic contents, all of which have antimalarial properties [26,29].
Despite its widespread presence on the Kenyan coast, our investigations revealed that no documented in-vivo research into the antiplasmodial efficacy of African A. marina has been conducted, resulting in a knowledge gap due to a lack of evidence-based data. As a result, the current study sought to identify phytochemicals as well as assess the safety and antiplasmodial efficacy of Kenyan Avicennia marina in a mice model.
Plant materials: stem barks of Avicennia marina were collected from Gazi bay mangrove ecosystem in Kwale County, coastal Kenya. The Department of Botany Herbarium at the University of Nairobi's, School of Biological Sciences performed the identification and authentication of the plant. For documentation, a voucher specimen was placed in the herbarium (voucher number JNM 2019/02). The barks were air dried for five days under shade.
Preparation of extracts: the extraction was done using procedure described by Okaiyeto et al. . Briefly, the dried stem barks were ground to powder using an electric miller. One hundred (100) gm of the powder was soaked in 500 mL of methanol for 48 hours at room temperature with frequent stirring in a 1 L volumetric flask. Whatman filter paper no. 1 was used to filter the mixture, and the filtrate was collected in a stoppered conical flask. For hot decoction, 100 g of the plant material was soaked in 500ml of distilled water and the mixture was boiled for 20 minutes. The mixture was filtered with Whatman filter paper no. 1 into a stoppered conical flask after being allowed to cool. Cold maceration was used to prepare the cold aqueous extract where 100 g of the powder was mixed with 500 ml of distilled water at room temperature. The mixture was extracted for 48 hours with continuous agitation using a magnetic stirrer. It was then filtered through Whatman filter paper no.1 and the filtrate collected in a 1 liter stoppered conical flask.
Concentration of the extracts: in order to get solid residue, the filtrates from organic solvents were concentrated in a rotary vacuum evaporator (buchi rotavapor R-205), and then freeze-dried (-20°C). Freeze-drying was used to concentrate the filtered aqueous extracts. Using the following formula, the extraction percentage yield was calculated :
Where W1 = the weight of the empty container; W2 = the weight of the container plus the weight of the plant extract; W0= the weight of the milled extract (100gm).
Phytochemical screening: a qualitative phytochemical analysis to identify important bioactive molecules such as steroids, coumarins, alkaloids, saponin, tannin, flavonoid, terpenoids, and anthraquinones was carried out using standard methodologies .
Experimental animals: a total of ninety (90) Swiss albino female mice weighing 18 to 22 grams and aged 5 to 6 weeks were procured from The Kenya Medical Research Institute´s (KEMRI's) animal house in order to conduct toxicity and antimalarial assays. They were housed in standard Macrolon type II cages for 12 hours at a constant room temperature of 25 to 27 degrees Celsius and a relative humidity of 60 to 70 percent, with unlimited access to food and water. Animals were moved from the unit to the laboratory at least 30 to 60 minutes before use in order to reduce the impact of stress on them. Permission and approval for animal use were obtained from the Kenya Medical Research Insitute Animals Care and Use Committee.
Experimental parasites and inoculation procedures: cryopreserved P. berghei, were obtained from KEMRI´s Center for Traditional Medicine Research (CTMDR) laboratory. Serial passage of P. berghei was initiated by administering 2 × 107 (0.2ml) of blood-containing parasites intraperitoneally (i.p.) into albino mice and allowing the rodents to develop the parasites for 7 days. While under anaesthesia induced by halothane in a closed chamber, 1 to 2 ml of blood was drawn via cardiac puncture into a heparinized vacutainer tube. Based on the parasitaemia level of the donor mice and the red blood cell (RBC) count of normal mice, blood was diluted with sterile, non-pyrogenic 0.85% physiological saline so that 1 ml of blood contains 5 × 107 infected red blood cell (RBC). Zero point two ml of this diluted blood, which contained 1 × 107 P. berghei-infected RBCs, was administered intraperitoneally into each test- and control-group mice .
In vivo acute toxicity test: in accordance with Organization for Economic Cooperation and Development (OECD) Guideline no. 425 , non-infected female Swiss albino mice were used to test the acute toxicity of crude methanol and acqueous extracts of avicennia marina on the animals. The mice were 5-6 weeks old and weighed 18-22 g. Five mice were fasted overnight but were allowed water ad libitum and were weighed before testing. Briefly, a single dose of 5000 mg/kg (0.2 ml) of each extract (aqueous and methanol) was given orally to a female mouse. Food was restricted for a further two hours after extract administration. The mouse was observed continuously for 30 minutes for 4 hrs and thereafter 24 hrs. Since no deaths were noted within the first 24 hours, the additional four mice were given the same dosage of the extract (5000 mg/kg) and monitored for any signs of toxicity over the course of the following 14 days, such as decreased respiration, writhing, reduced motor activity, reduced body tone, and death.
Pharmacological screening (Peter´s four-day suppressive test): the schizontocidal activity of the crude extract against chloroquine-sensitive P. berghei-infected mice was assessed using Peter's 4-day suppression test . Briefly, female Swiss albino mice weighing 18-22 g were intraperitoneally injected with 0.2 ml of infected blood on the first day (day 0). The mice were then randomly divided into six groups of five mice each. Groups I, II, III and IV were designated as test groups while groups V and VI were designated negative and positive control groups, respectively. The test groups received 0.2 mls of 100 mg/kg, 250 mg/kg, and 500 mg/kg crude extract of A. marina via oral gavage three hours after parasites inoculation, while the positive and negative control groups received chloroquine at a dose of 10 mg/kg and an equivalent amount of vehicle (0.2 ml distilled water) respectively for four consecutive days (day 0-3). A drop of blood was taken from each mouse's tail on the fifth day (day 4, 24 hours after the last dose, or 96 hours after infection), and it was used to make thin blood smears that were stained with 10% Giemsa. And by counting the number of parasitized erythrocytes in five randomly selected fields of a microscope using an oil immersion objective (100x magnification), the following equation was used to determine the parasitemia level :
Average chemo-suppression by percentage was determined as :
Where A represents the average parasitaemia in the test group and B represents the average parasitaemia in the negative control group, respectively.
Rane´s (curative) test: according to Nardos et al. procedures , the crude extract's chemotherapeutic effectiveness against an already-established illness was evaluated. Briefly, mice were intraperitoneally injected with 0.2 ml of a standard inoculum containing 1 × 107 P. berghei-infected erythrocytes on day 0 of the experiment. At day 3, mice were randomly assigned to two groups of positive and negative control (V and VI), and four test groups (I, II, III, and IV), each group with five mice. Positive and negative controls were given oral doses of 0.2 ml of 10 mg/kg of chloroquine and vehicle (distilled water), respectively. Each mouse's tail was processed into a thin blood film, air dried, and Giemsa stained on days 3 and 7 to track the parasitaemia level.
Prophylactic (repository infection) test: the procedure described by Peters  was modified slightly to examine the crude extracts' prophylactic activities against residual infection. Six groups of five mice each were randomly selected from the mouse population. Animals in group I (negative control) and II (positive control) were given 0.2 mls of vehicle (distilled water) and 0.2 ml (10 mg/kg) of chloroquine respectively. Exactly 0.2 ml of 100 mg/kg, 250 mg/kg, or 500 mg/kg of the extract were given orally to groups II through V. The mice in each group received treatment for 3 days in a row (D0 - D2), then on day 4 (D3), the 0.2ml infected blood was intraperitoneally injected into the mice. Within 72 hours of inoculation, thin films were made from the tail blood of each mouse, Giemsa stained to assess the parasite density.
Monitoring mean survival time: in all of the in vivo antiplasmodial experiments, mortality was tracked and the number of days from the moment of parasite inoculation up until death was recorded for each mouse in the treatment and control groups for 30 days. Each group's mean survival time (MST) was calculated as follows :
Monitoring body weight: each mouse's body weight was recorded on days 0 (D0) and 4 (D4) of the 4-day suppressive test, one hour prior to infection on D0 and following treatment on D4, respectively. For Rane´s and repository test the body weight for each mouse was taken 3 hours before infection on day-0 (D0) and after treatment on day-7 (D7) to determine the efficacy of methanolic and aqueous crude extracts of A. marina stem bark on P. berghei infected mice :
Data quality control: the experimental animals were randomly selected and marked with a permanent marker pen to permit individual identification. During preparation of blood smears, unique codes were assigned in frosted microscopic slide. Parasites count was done by 4 blinded experienced laboratory technicians.
Data analysis: data were analyzed using SPSS version 21.0 software and results of the study were expressed as a mean ± standard error of the mean (M ± SEM). One-way analysis of variance (ANOVA) was used to compare between multiple treatment groups followed by Scheffe´s and Tamhane´s T2 post hoc test. P-values less than 0.05 were regarded as significant.
Ethical clearance: approval to use animal models was sort from Animal Care and Use Committee (KEMRI-ACUC). Ethical review and approval was obtained from the Scientific Ethics Review Unit-Kenya Medical Research Institute (KEMRI/RES/7/3/1).
Extract yields: stem bark extracts yielded a total of 23.1 g (23.1%) and 21.82 g (21.82%) of dried aqueous crude extract and dried methanolic crude extract respectively.
Phytochemical analysis: Aviccenia marina's stem bark extracts were subjected to a qualitative phytochemical screening test, which revealed that the plant contains alkaloids, anthraquinones, anthrocyanides, tannins, saponins, triterpenoids, flavanoids, and cardiac glycosides but no phenols or steroids (Table 1).
Acute cytotoxicity: in the in vivo acute toxicity test no mortality was observed between day 0 and day 24 h and day 14 following oral treatment with either aqueous or methanolic extracts of A. marina stem bark at a dose of 5000 milligrams per kilogram of body weight. Additionally, no overt poisoning symptoms such writhing, reduced motor activity, decreased body tone, decreased feeding behaviors, or decreased limb tone were noticed. Lethal dose fifty (LD50) was therefore assumed to be above 5000 mg/kg.
Pharmacologic screening (Peter´s 4-day suppressive) test: the findings of the 4-day suppressive test revealed that both aqueous and methanolic extracts had exceptional antiplasmodial action against female Swiss albino mice infected with chloroquine susceptible P. berghei as shown in Figure 1. Dose-dependent antischizoidal ability was demonstrated by all doses. The degree of suppression by aqueous extract after the 4-day test at dosages of 100, 250, and 500 mg/kg was 39.2%, 88.53%, and 90.20%, respectively, and that of methanol extract was 38.18%, 92.60% and 93%, respectively. Both aqueous and methanol extracts significantly (p<0.05) reduced parasitemia compared to negative control. At higher doses of 500 mg/kg, both methanolic and aqueous extracts showed significantly (p<0.001) higher percentage suppression of 93% and 90.21%, respectively compared to that of chloroquine (CQ) (10 mg/kg) (99.33%). The survival duration of the infected mice was significantly (p<0.05) extended by both the aqueous and methanolic stem bark extracts as compared to the negative control group (Figure 2).
Effect of suppressive test on body weight of infected mice: as shown in Table 2, none of the extract doses significantly (p>0.05) prevented mice from body weight loss in comparison to the negative control animals. However, the conventional medication (chloroquine 10 mg/kg) significantly (p<0.001) prevented body weight loss related to malaria compared to control mice.
Rane´s (curative) test: when compared to the group that received a vehicle treatment, both aqueous and methanolic crude extracts significantly (p<0.001) reduced parasitemia in a dose-dependent manner (Figure 3). In comparison to the negative control, survival time was significantly (p<0.05) prolonged in all doses of the test sample (Figure 4). However, compared to CQ 10mg/kg, the extracts' therapeutic impact was less potent.
Effects of curative test on body weight of infected mice: in comparison to the negative control, all doses significantly (p<0.05) prevented the mice from losing body weight (Table 3). The body weight gain, however, was not dose-dependent.
The prophylactic (repository) test: when compared to the negative control, all doses of aqueous and methanolic crude extracts significantly (p<0.001) reduced parasite burden (Figure 5). Although total parasite suppression was not achieved, methanol (MeOH) 500mg/kg showed the highest levels of parasitemia suppression (87.64%) among the experiments. At all doses of the aqueous and methanolic extracts of the stem barks of A. marina, there was a significant (p<0.05) difference in the mean survival time between test mice and the negative control group (Figure 6).
Effects of prophylactic test on body weight of infected mice: when compared to the vehicle in all doses, methanolic crude extracts significantly (p<0.001) reduced weight loss in infected mice. When compared to the vehicle group, weight loss in test mice was significantly (p<0.01) reduced by both the aqueous extract (AE) 250mg/kg and AE 500mg/kg extracts. Aqueous extract (AE) 100mg/kg extract, however, was unable to prevent the test mice from losing weight (Table 4).
For decades, nature has been the source of therapeutic agents, and based on their traditional use, new drugs have been discovered from natural sources . Due to accelerating rate in drug resistance of common drugs in malaria endemic regions, the necessity to find and create alternative antimalarial medications is unavoidable . The choice of A. marina plant was informed by previous reports of their antiplasmodial activities [21,34,37]. The phytochemical screening of the extracts of A. marina, a mangrove plant found along coast line of Kenya revealed presence of several bioactive compounds with antiplasmodial activities; flavanoids, tannins, saponin, triterpenoids, glycosides, anthraquinones and alkaloids. These metabolites were also reported in similar studies [47,48]. Similar phytochemicals have also been found in other plant extracts which exhibited antimalarial activity [49,50].
Absence of phenolic and steroid compounds contradicts similar study done in Egypt which reported their presence . Also, the presence of saponin in the present study contradicts a previous study conducted elsewhere which reported absence of saponin . Similarly, anthraquinones and anthocyanine found in the present study were not found in a similar study . The differences in the quantity and composition of secondary metabolites in plants may be influenced by a variety of abiotic environmental factors, including geographic factors (latitude, altitude), sunlight, and climatic, edaphic, and biotic interactions . Additionally, plant developmental stage, post-harvest handling, such as duration of storage, exposure to light, pest and pathogen attack, and storage temperature, are variables that may influence phytochemical variations .
Although rodents malaria do not produce exact signs and symptoms as observed in human plasmodial infections, Plasmodium berghei infections in mice are reported to cause pathological signs and symptoms akin to human malaria . Some of the available anti-malarial drugs (halofantrine, chloroquine, mefloquine, and artemisinin derivatives) have been identified through use of in vivo models . In-vivo antiplasmodial activity models were used in this study because they account for prodrug effects as well as the immune system's potential role in infection eradication . Therefore, Swiss albino mice infected with chloroquine sensitive P. berghei were used as experimental model to test the antiplasmodial efficacy of stem bark extracts of A. marina.
The fact that no mortality was observed at the highest oral dose of 5000 mg/kg body weight of the aqueous and methanolic stem bark extracts of A. marina indicated that the mice were safe at the high limit dose as recommended by Organization for Economic Cooperation and Development (OECD) guideline no 425 on acute toxicity . Similar safety findings were reported on similar plant [56,57]. The aqueous and methanolic stem bark extracts of A. Africana, a species of the plant that is closely related, produced similar safety results . However, it will be difficult to extrapolate the safety to humans due to species variations .
Three models (4-day suppressive, curative and prophylactic tests) are primarily used to test the antiplasmodial effects of the plant . When compared to the negative control group, crude extracts significantly (p<0.05) reduced the early (schizont) stage development of P. berghei. The group treated with MeOH500 mg/kg experienced the maximum suppression (93%); this finding suggests that active metabolites increase as dose is increased. According to a prior in vitro investigation on the same plant, the extracts suppressed growth at higher concentrations, and our result is consistent with that finding . Given the closeness of suppression levels in aqueous and methanolic extracts at 250 mg/kg and 500 mg/kg, it is possible that the 250 mg/kg dose is the optimal therapeutic dose in mice .
Both aqueous and methanolic extracts significantly (p<0.001) reduced parasitemia in the curative test for established infection dose dependently as compared to the negative control. The highest suppression occurred in MeOH 500mg/kg (63.25%). A good prophylactic activity of both aqueous and methanolic A. marina extracts was observed in the rodent malaria model. In this study, the 100 mg/kg dose may be the ideal therapeutic dose in mice based on the proximity of suppression in curative and prophylactic testing values at 100 mg/kg, 250 mg/kg, and 500 mg/kg body weight. This is similar to the research conducted by Alli et al. .
Our study's significant (p<0.05) dose-dependent suppression, curative, and prophylactic effects of A. marina are consistent with other studies [50,61]. Neither the conventional medication (CQ 10mg/kg) nor the crude extracts entirely eliminated the parasitemia. This might be due to recrudescence potential of P. berghei parasites or short half-lives of some bioactive compounds [62,63]. A previous in vitro study elsewhere showed that leaf and bark extracts of A. marina has significant antiplasmodial activity . To the best of our knowledge, a similar in vivo study on the antiplasmodial efficacies of A. marina has not been documented, thus making this the first one.
Potential antiplasmodial agent is considered as moderate, good and very good, if the suppression is ≥ 50% at 500 mg/kg, 250 mg/kg and 100 mg/kg body weight, respectively . Based on this criterion, both aqueous and methanolic extracts from A. marina exhibited good activities in curative and prophylactic tests. Moderate activities were observed in the suppressive test. Moderately efficacious herbs are known to lessen the risks of mortality, alleviate malaria symptoms such as fever and pain, and abate the duration of illness by reducing anaemia as well as boosting the host´s immune system thus acting as immunomodulators . Different studies have showed promising antiplasmodial activity of mangrove species A. germinan, A. officinalis, and A. Africana that are closely related to A. marina [26,50,64,66,67].
The antiplasmodial activities of the extracts could be attributed to different effects; individually or in synergy with bioactive compounds such as tannins, alkaloids, terpenoids, steroids, tannins, anthraquinones and flavonoids [43,68]. Alkaloids exhibits antiplasmodial activities by blocking protein synthesis in P. falciparum . Quinine, one of the first and most effective antimalarial medications, is an example of an alkaloid and is derived from cinchona bark . Flavonoids act by chelating the parasite nucleic acid base pairing . Tannins inactivate microbial adhesins and cell envelope transport proteins . Anthraquinones molecules intercalate into deoxyribonucleic acid (DNA) of microbes, stabilize the covalent intermediate complex of the topo II reaction, causing cell death through apoptosis . Antiplasmodial effects of terpenoids and their derivatives are linked to endoperoxidation of microbes . Likewise, antioxidant properties of bioactive compounds has also been found to be responsible for antiplasmodial activity [41,73]. Antioxidant metabolites impedes the polymerization of heme, which is lethal for the intra-erythrocyte parasite . Indirect immune modulations and other target pathways which are not fully elucidated might also be responsible for parasite suppression [19,75].
Reduction in body weight is a typical feature of malaria in mice, thus a good antimalarial agent should protect plasmodium infected mice against weight loss . When compared to the negative control, the crude extracts failed to significantly (p>0.05) shield mice from weight loss brought on by the malaria parasite in the suppressive test. This might be explained by metabolic processes and hypoglycemia brought on by malaria infection . But when compared to a negative control, extracts from curative and preventative tests significantly (p<0.05) prevented weight loss in the sick mice. The parasite clearance in treated animals, the higher calorie intake, and the enhanced metabolite-related metabolic performance could all have contributed to this .
The anti-plasmodial effectiveness of plant extracts is also assessed using the mean survival time . The extracts in suppressive, curative and prophylactic tests significantly (p<0.05) increased the survival time of the experimental mice compared to the negative control group. In another investigation, similar finding was made . This demonstrates the extracts' capacity for protection as the plant repressed P. berghei and reduced the parasite's overall pathological consequences in the mice .
The study found that stem bark extracts of Avicennia marina significantly reduced Plasmodium berghei activity in a mice model. Furthermore, extracts of this plant at tested doses posed no health risk, supporting the use of these compounds in the treatment of rodent malaria. However, studies with human Plasmodium strains are suggested.
What is known about this topic
- Avicennia marina is a mangrove plant specie;
- Studies done in some Asian and African countries have shown that it prossesses chemotherapeutic properties.
What this study adds
- Our finding points to the possible presence of potentially effective antiplasmodial constituents in the stem bark of Kenyan Avicennia marina that suppress parasitemia in vivo models;
- Our findings may serve as the foundation for further investigation of Avicennia marina's antiplasmodial compounds.
The authors declare no competing interests.
Designing, data analysis, interpretation, and manuscript preparation were done by all of the authors. They also read and approved the final version of this manuscript.
Centre for Traditional Medicine Research, Nairobi, Kenya is highly acknowledged for providing research facilities. The authors are grateful to KEMRI´s animal house workers Stephen Kaniaru and Mary for their technical assistance.
Table 1: phytochemicals of A. marina extracts
Table 2: body weight change of infected mice treated with Avicennia marina extract in the suppressive test
Table 3: body weight change of infected mice treated with Avicennia marina extract in the curative test
Table 4: body weight change of infected mice treated with Avicennia marina extract in the prophylactic test
Figure 1: effects of A. marina crude extracts on parasitemia suppression of P. berghei infected mice in the 4-day suppressive test
Figure 2: effects of A. marina crude extracts on mean survival time of P. berghei infected mice in the 4-day suppressive test
Figure 3: effects of A. marina crude extracts on parasitemia suppression of P. berghei infected mice in the curative test
Figure 4: effects of A. marina crude extracts on mean survival time of P. berghei infected mice in the curative test
Figure 5: effects of A. marina crude extracts on parasitemia suppression of P. berghei infected mice in the prophylactic test
Figure 6: effects of A. marina crude extracts on mean survival time of P. berghei infected mice in the prophylactic test
- Gebrehiwot S, Shumbahri M, Eyado A, Yohannes T. Phytochemical Screening and In Vivo Antimalarial Activity of Two Traditionally Used Medicinal Plants of Afar Region, Ethiopia, against Plasmodium berghei in Swiss Albino Mice. J Parasitol Res. 2019 Jun 2;2019:4519298. PubMed | Google Scholar
- Sultana M, Sheikh N, Mahumud RA, Jahir T, Islam Z, Sarker AR. Prevalence and associated determinants of malaria parasites among Kenyan children. Trop Med Health. 2017;45:25. PubMed | Google Scholar
- Tse EG, Korsik M, Todd MH. The past, present and future of anti-malarial medicines. Malar J. 2019;18(1):93. PubMed | Google Scholar
- World Health Organization (WHO). World malaria report 2019. 2019. Accessed 13th February, 2023.
- Ministry of Health Division of National Malaria Programme Nairobi, Kenya,Kenya National Bureau of Statistics Nairobi, Kenya, The DHS Program Rockville, Maryland, USA. Kenya Malaria Indicator Survey 2020: Final Report. 2021. Accessed 13th February, 2023.
- Kucharski DJ, Jaszczak MK, Boratynski PJ. A Review of Modifications of Quinoline Antimalarials: Mefloquine and (hydroxy) Chloroquine. Molecules. 2022 Feb 2;27(3):1003. PubMed | Google Scholar
- World Health Organization. Guidelines for the Treatment of Malaria. 3rd edition. Geneva: World Health Organization. 2015. PubMed | Google Scholar
- Pothula VVS, Kanikaram S. In vitro antiplasmodial efficacy of mangrove plant, Ipomoea pes-caprae against Plasmodium falciparum (3D7 strain). Asian Pacific J Trop Dis. 2015;5(12):947-56. Google Scholar
- World Health Organization. Malaria entomology and vector control. World Heal Organ. 2013;192. Google Scholar
- Wanjala CL, Kweka EJ. Malaria Vectors Insecticides Resistance in Different Agroecosystems in Western Kenya. Front Public Health. 2018 Mar 1;6:55. PubMed | Google Scholar
- Omara T. Antimalarial Plants Used across Kenyan Communities. Evid Based Complement Alternat Med. 2020 Jun 12;2020:4538602. PubMed | Google Scholar
- Dondorp AM, Nosten F, Yi P, Das D, Phyo AP, Tarning J et al. Artemisinin resistance in Plasmodium falciparum malaria. N Engl J Med. 2009 Jul 30;361(5):455-67. PubMed | Google Scholar
- Imwong M, Suwannasin K, Kunasol C, Sutawong K, Mayxay M, Rekol H et al. The spread of artemisinin-resistant Plasmodium falciparum in the Greater Mekong subregion: a molecular epidemiology observational study. Lancet Infect Dis. 2017 May;17(5):491-497. PubMed | Google Scholar
- Balikagala B, Fukuda N, Ikeda M, Katuro OT, Tachibana SI, Yamauchi M et al. Evidence of Artemisinin-Resistant Malaria in Africa. N Engl J Med. 2021 Sep 23;385(13):1163-1171. PubMed | Google Scholar
- Uwimana A, Legrand E, Stokes BH, Ndikumana JM, Warsame M, Umulisa N et al. Emergence and clonal expansion of in vitro artemisinin-resistant Plasmodium falciparum kelch13 R561H mutant parasites in Rwanda. Nat Med. 2020 Oct;26(10):1602-1608. PubMed | Google Scholar
- Gelband H, Panosian CB, Arrow KJ, editors. Saving Lives, Buying Time: Economics of Malaria Drugs in an Age of Resistance. Washington (DC): National Academies Press (US). 2004. PubMed | Google Scholar
- Medoatinsa SE, Dossa CA, Viwami F, Bogninou-Agbidinoukoun GS, Noudogbessi JP, Lagnika L et al. In Vitro Antiplasmodial and Antioxidant Activities of Ethanolic and Hydroethanolic Extracts of Hyptis Suaveolens. World J Pharm Pharm Sci. 2015;4(8):101-12. Google Scholar
- Shibeshi MA, Enyew EF, Adinew GM, Aragaw TJ. Antimalarial Activity of Methanolic Extracts and Solvent Fractions of Combretum molle Leaves in Plasmodium berghei Infected Mice. J Exp Pharmacol. 2021;13:69-89. PubMed | Google Scholar
- Wilmot D, Ameyaw EO, Amoako-Sakyi D, Boampong JN, Quashie NB. In vivo efficacy of top five surveyed Ghanaian herbal anti - malarial products. Malar J. 2017 Mar 4;16(1):103. PubMed | Google Scholar
- Bekono BD, Ntie-Kang F, Onguéné PA, Lifongo LL, Sippl W, Fester K et al. The potential of anti-malarial compounds derived from African medicinal plants: a review of pharmacological evaluations from 2013 to 2019. Malar J. 2020;19(1):183. PubMed | Google Scholar
- Muhaimin M, Latief M, Putri RD, Chaerunisaa AY, Aditama AY, Pravitasari NE et al. Antiplasmodial Activity of Methanolic Leaf Extract of Mangrove Plants against Plasmodium berghei. Pharmacogn J. 2019;11(5):929-35. Google Scholar
- Deborah AF, Aanuoluwapo O, Ebunoluwa F. Comparative in vivo antiplasmodial activities of different extracts of Lawsonia inermis, Tithonia diversifolia and Nauclea latifolia against Plasmodium berghei. African J Biol Sci. 2020;2(1):9-7. Google Scholar
- Muthaura CN, Rukunga GM, Chhabra SC, Mungai GM, Njagi EN. Traditional antimalarial phytotherapy remedies used by the Kwale community of the Kenyan Coast. J Ethnopharmacol. 2007 Dec 3;114(3):377-86. PubMed | Google Scholar
- Otieno NE, Analo C. Local indigenous knowledge about some medicinal plants in and around Kakamega forest in western Kenya. F1000Res. 2012 Oct 31;1:40. PubMed | Google Scholar
- Salini G. Pharmacological profile of mangrove endophytes-a review. Int J Pharm Pharm Sci. 2015;7(1):6-15
- Bandaranayake WM. Bioactivities, bioactive compounds and chemical constituents of mangrove plants. Wetl Ecol Manag. 2002;10:421-52. Google Scholar
- Beula JM, Gnanadesigan M, Rajkumar PB, Ravikumar S, Anand M. Antiviral, antioxidant and toxicological evaluation of mangrove plant from South East coast of India. Asian Pac J Trop. 2012;2:S352-7. Google Scholar
- Liao S, Wang Y, Liu H, Fan G, Sahu SK, Jin T et al. Deciphering the Microbial Taxonomy and Functionality of Two Diverse Mangrove Ecosystems and Their Potential Abilities To Produce Bioactive Compounds. mSystems. 2020 Oct 27;5(5):e00851-19. PubMed | Google Scholar
- Thatoi H, Samantaray D, Das SK. The genus Avicennia, a pioneer group of dominant mangrove plant species with potential medicinal values: a review. Front Life Sci. 2016;9(4):267-91. Google Scholar
- Sugga A, Elageed S, Rajakhan O, Bashair A. Phytochemial screening and anti-microbial activity of Mangrove Gum against some Pathogens. 2014;1-14. Google Scholar
- Takarina ND, Arif GA, Juhriah SA. Phytochemical contents and antioxidant activities of mangrove (Avicennia marina) leaves extract. InAIP Conference Proceedings. 2018. Google Scholar
- Abou Seedo K, Abido MS, Salih A, Abahussain A. Morphophysiological Traits of Gray Mangrove (Avicennia marina (Forsk.) Vierh.) at Different Levels of Soil Salinity. Int J For Res. 2018;2018:7404907. Google Scholar
- Wang’ondu VW, Kairo JG, Kinyamario JI, Mwaura FB, Bosire JO, Dahdouh-Guebas F et al. Phenology of Avicennia marina (Forsk.) Vierh. in a Disjunctly-zoned Mangrove Stand in Kenya. West Indian Ocean J. 2010;9:135-44. Google Scholar
- Ravikumar S, Inbaneson SJ, Suganthi P, Gnanadesigan M. In vitro antiplasmodial activity of ethanolic extracts of mangrove plants from South East coast of India against chloroquine-sensitive Plasmodium falciparum. Parasitol Res. 2011 Apr;108(4):873-8. PubMed | Google Scholar
- Okaiyeto K, Okoh AI. In vitro assessment of antiplasmodial and antitrypanosomal activities of chloroform, ethyl acetate and ethanol leaf extracts of Oedera genistifolia. Appl Sci. 2020;10(19):6987. Google Scholar
- Abubakar AR, Haque M. Preparation of Medicinal Plants: Basic Extraction and Fractionation Procedures for Experimental Purposes. J Pharm Bioallied Sci. 2020 Jan-Mar;12(1):1-10. PubMed | Google Scholar
- Mouafi FE, Abdel-aziz SM, Bashir AA, Fyiad AA. Phytochemical Analysis and Antimicrobial Activity of Mangrove Leaves (Avicenna marina and Rhizophora stylosa) Against Some Pathogens Department of Marine Biology, Faculty of Marine and Science Fisheries. World Appl Sci J. 2014;29:547-54.
- Basir R, Rahiman SF, Hasballah K, Chong W, Talib H, Yam M et al. Plasmodium berghei ANKA Infection in ICR Mice as a Model of Cerebral Malaria. Iran J Parasitol. 2012;7(4):62-74. PubMed | Google Scholar
- Organization for Economic Cooperation and Development (OECD). Test No. 425: Acute Oral Toxicity: Up-and-Down Procedure. 2008.
- Peters W, Portus JH, Robinson BL. The chemotherapy of rodent malaria, XXII. The value of drug-resistant strains of P. berghei in screening for blood schizontocidal activity. Ann Trop Med Parasitol. 1975 Jun;69(2):155-71. PubMed | Google Scholar
- Bantie L, Assefa S, Teklehaimanot T, Engidawork E. In vivo antimalarial activity of the crude leaf extract and solvent fractions of Croton macrostachyus Hocsht. (Euphorbiaceae) against Plasmodium berghei in mice. BMC Complement Altern Med. 2014 Mar 1;14:79. PubMed | Google Scholar
- Bonkian LN, Yerbanga RS, Koama B, Soma A, Cisse M, Valea I et al. In Vivo Antiplasmodial Activity of Two Sahelian Plant Extracts on Plasmodium berghei ANKA Infected NMRI Mice. Evid Based Complement Alternat Med. 2018 May 24;2018:6859632. PubMed | Google Scholar
- Nardos A, Makonnen E. In vivo antiplasmodial activity and toxicological assessment of hydroethanolic crude extract of Ajuga remota. Malar J. 2017 Jan 13;16(1):25. PubMed | Google Scholar
- Peters W. Drug resistance in Plasmodium berghei Vincke and Lips, 1948. I. Chloroquine resistance. Exp Parasitol. 1965 Aug;17(1):80-9. PubMed
- Kifle ZD, Adinew GM, Mengistie MG, Gurmu AE, Enyew EF, Goshu BT et al. Evaluation of Antimalarial Activity of Methanolic Root Extract of Myrica salicifolia A Rich (Myricaceae) Against Plasmodium berghei-Infected Mice. J Evid Based Integr Med. 2020;25:2515690X20920539. PubMed | Google Scholar
- White NJ. Antimalarial drug resistance. J Clin Invest. 2004 Apr;113(8):1084-92. PubMed | Google Scholar
- Moghal MM, Bhattacharjee A, Seeam SM, Islam AM, Bappy MH. Phytochemical Screening, Cytotoxic and Anthelmintic Activities of Amorphophallus campanulatus (Roxb.), Avicennia marina (Forssk.) and Launaea sarmentosa (Willd.). Bangladesh Pharm J. 2016;19(1):106-13. Google Scholar
- Ibrahim HAH, Abdel-Latif HH, Zaghloul EH. Phytochemical composition of Avicennia marina leaf extract, its antioxidant, antimicrobial potentials and inhibitory properties on Pseudomonas fluorescens biofilm. Egypt J Aquat Res. 2022;48(1):29-35. Google Scholar
- Gboeloh LB, Okon OE, Udoh SE. Antiplasmodial Effect of Anthocleista vogelii on Albino Mice Experimentally Infected with Plasmodium berghei berghei (NK 65). J Parasitol Res. 2014;2014:731906. PubMed | Google Scholar
- Ahmed MA, Ameyaw EO, Ackah-Armah F, Acheampong DO, Amoani B, Ampomah P et al. In vitro and In vivo antimalarial activities of Avicennia africana P. Beauv. (Avicenniaceae) ethanolic leaf extract. J Tradit Complement Med. 2021 Dec 3;12(4):391-401. PubMed | Google Scholar
- Mohammadi Bazargani M, Falahati-Anbaran M, Rohloff J. Comparative Analyses of Phytochemical Variation Within and Between Congeneric Species of Willow Herb, Epilobium hirsutum and E. parviflorum: Contribution of Environmental Factors. Front Plant Sci. 2021 Feb 17;11:595190. PubMed | Google Scholar
- Raya KB, Ahmad SH, Farhana SF, Mohammad M, Tajidin NE, Parvez A. Changes in phytochemical contents in different parts of Clinacanthus nutans (Burm. f.) lindau due to storage duration. Bragantia. 2015;74:445-52. Google Scholar
- Okokon JE, Mobley R, Edem UA, Bassey AI, Fadayomi I, Drijfhout F et al. In vitro and in vivo antimalarial activity and chemical profiling of sugarcane leaves. Sci Rep. 2022;12(1):10250. PubMed | Google Scholar
- Belay WY, Endale Gurmu A, Wubneh ZB. Antimalarial Activity of Stem Bark of Periploca linearifolia during Early and Established Plasmodium Infection in Mice. Evid Based Complement Alternat Med. 2018 Jan 29;2018:4169397. PubMed | Google Scholar
- Jiménez-Díaz MB, Viera S, Ibáñez J, Mulet T, Magán-Marchal N, Garuti H et al. A new in vivo screening paradigm to accelerate antimalarial drug discovery. PLoS One. 2013;8(6):e66967. PubMed | Google Scholar
- Ali BH, Bashir AK. Toxicological studies on the leaves of Avicennia marina (mangrove) in rats. J Appl Toxicol. 1998;18(2):111-6. PubMed | Google Scholar
- Sukhramani PS, Patel PM. Biological screening of Avicennia marina for anticancer activity. Der Pharm Sin. 2013;4:125-30. Google Scholar
- Budka A, Lacka A, Szoszkiewicz K. The use of rarefaction and extrapolation as methods of estimating the effects of river eutrophication on macrophyte diversity. Biodivers Conserv. 2019;28:385-400. Google Scholar
- Teklu T, Engidawork E, Nedi T, Teklehaymanot T, Gebremeskel L. Evaluation of the Antimalarial Activity of the Hydroalcoholic Extract of Leaf of Leonotis ocymifolia (Burm. f.) Iwarsson (Lamiaceae) against Plasmodium berghei in Mice. Evid Based Complement Alternat Med. 2020;2020:5384804. PubMed | Google Scholar
- Alli LA, Adesokan AA, Salawu OA, Akanji MA, Tijani AY. Anti-plasmodial activity of aqueous root extract of Acacia nilotica. African J Biochem Res. 2011;5(7):214-9. Google Scholar
- Das SK, Samantaray D, Thatoi H. Ethnomedicinal, Antimicrobial and Antidiarrhoeal Studies on the Mangrove Plants of the Genus Xylocarpus: A Mini Review. J Bioanal Biomed. 2014;12(004).
- Fentahun S, Makonnen E, Awas T, Giday M. In vivo antimalarial activity of crude extracts and solvent fractions of leaves of Strychnos mitis in Plasmodium berghei infected mice. BMC Complement Altern Med. 2017;17(1):13. PubMed | Google Scholar
- Habte G, Assefa S. In Vivo Antimalarial Activity of Crude Fruit Extract of Capsicum frutescens Var Minima (Solanaceae) against Plasmodium berghei-Infected Mice. Biomed Res Int. 2020;2020:1320952. PubMed | Google Scholar
- Ravikumar S, Inbaneson SJ, Suganthi P, Venkatesan M, Ramu A. Mangrove plants as a source of lead compounds for the development of new antiplasmodial drugs from South East coast of India. Parasitol Res. 2011 Jun;108(6):1405-10. PubMed | Google Scholar
- Walter N, Gorki V, Bagai U. Antiplasmodial potential of Thalictrum foliolosum (Ranunculaceae) against lethal murine malaria. J Vector Borne Dis. 2020;57(3):204-12. PubMed | Google Scholar
- Bandaranayake WM. Traditional and medicinal uses of mangroves. Mangroves Salt Marshes. 1998;2:133-48. Google Scholar
- Khafagi I, Gab-Alla A, Salama W, Fouda M. Biological activities and phytochemical constituents of the gray mangrove Avicennia marina (Forssk.). Egypt J Biol. 2004;5:62-9. Google Scholar
- Nadia NAC, Cédric Y, Christian NO, Raoul SNS, Jeanette Y, Abdel Azizi M et al. In Vivo Antiplasmodial Activity of Entandrophragma cylindricum (Sprague) Sprague Ethyl Acetate Extract in Plasmodium berghei-Infected Mice. J Parasitol Res. 2020;2020:8846067. PubMed | Google Scholar
- Johnson IS, Ettebong EO, Okokon JE. In vivo antiplasmodial activities of ethanolic leaf extract and fractions of Hilleria latifolia. J Med Plants Stud. 2017;5(4):118-22. Google Scholar
- Keita S, Wélé M, Cisse C, Diarra N, Kirkman L, Baba-Moussa L. Antibacterial and Antiplasmodial Activities of Tannins Extracted from Zizyphus mauritiana in Mali. Int J Biochem Res Rev. 2018;24(2):1-8. Google Scholar
- Diaz-Muñoz G, Miranda IL, Sartori SK, de Rezende DC, Diaz MAN. Anthraquinones: An Overview. Stud Nat Prod Chem. 2018;58:313-38. Google Scholar
- Okokon JE, Antia BS, Mohanakrishnan D, Sahal D, Okokon JE, Antia BS et al. Antimalarial and antiplasmodial activity of husk extract and fractions of Zea mays. Pharm Biol. 2017 Dec;55(1):1394-1400. PubMed | Google Scholar
- Okokon JE, Augustine NB, Mohanakrishnan D. Antimalarial, antiplasmodial and analgesic activities of root extract of Alchornea laxiflora. Pharm Biol. 2017 Dec;55(1):1022-1031. PubMed | Google Scholar
- Kouliga KB, Serge YR, Ollo DA, Sibidou YO, Magloire NH, Anicet OG et al. In Vivo Antimalarial Activity, Safety and Phytochemical Screening of Canthium Multiflorum (Schumach. Thonn.) Hiern (Rubiaceae). J Med Plants Res. 2022;16(6):196-204. Google Scholar
- Odugbemi TO, Akinsulire OR, Aibinu IE, Fabeku PO. Medicinal plants useful for malaria therapy in Okeigbo, Ondo State, Southwest Nigeria. Afr J Tradit Complement Altern Med. 2006 Nov 13;4(2):191-8. PubMed | Google Scholar
- Kabiru AY, Ibikunle GF, Innalegwu DA, Bola BM, Madaki FM. In Vivo Antiplasmodial and Analgesic Effect of Crude Ethanol Extract of Piper guineense Leaf Extract in Albino Mice. Scientifica (Cairo). 2016;2016:8687313. PubMed | Google Scholar
- Misganaw D, Amare GG, Mengistu G. Chemo Suppressive and Curative Potential of Hypoestes forskalei Against Plasmodium berghei: Evidence for in vivo Antimalarial Activity. J Exp Pharmacol. 2020;12:313-23. PubMed | Google Scholar
- Ryley JF, Peters W. The antimalarial activity of some quinolone esters. Ann Trop Med Parasitol. 1970 Jun;64(2):209-22. PubMed | Google Scholar