Giant mixed Sertoli-Leydig-Granulosa sex cord tumor of the testis; clinical,
histopathological, and radiological features: a case report
Sakher Tahaineh1, Rawan Abu Mughli2, Moayid Fallatah3,&
1Urology Department, Security Forces Hospital, Makkah, Saudi Arabia, 2Radiology
Department, Security Forces Hospital, Makkah, Saudi Arabia, 3College
of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia
Moayid Fallatah, College of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia
Sex cord tumors of the testis in post pubertal men are rare. Mixed leydig-Sertoli-Granulosa
sex cord tumors are exceptionally rare. To the best of our knowledge there
are only three reported similar cases in the literature. We reported a
case of a 27-year-old male who presented with huge left scrotal mass of
6-years duration. The gross tumor specimen after resection measured 11
cm in diameter.
Histological examination revealed mixed sex cord stromal tumor. This case
demonstrates the limited ability of accurate diagnostic determination preoperatively,
with pathologic examination and immune-histochemical staining post-orchiectomy
representing the only definitive means of diagnosis. It also highlights
the unique radiological appearances of this tumor, which were not previously
Sex cord tumors of the testis in post pubertal men are rare [1,2].
Mixed leydig-Sertoli-Granulosa sex cord tumors, are exceptionally rare,
with only three reported cases in literature [1,3,4].
We report a case of a 27-year-old male with a huge mixed sex cord tumor of testis,
which has a unique presentation of a long standing left scrotal mass
of 6-years duration. An outline of this case clinical, radiological and pathological
Patient and observation
A 27-year-old man presented to our urology clinic with left testicular
swelling of 6-years duration. His medical history was negative,
particularly for cryptorchidism. On physical examination, there
was a large non tender
swelling of the left scrotum, which showed partial transillumination.
There was no gynecomastia or any systemic symptoms. Accordingly,
examination was done; this showed large mixed solid and multilocular
cystic mass replacing the left testis. The cysts vary in appearance
to echogenic (Figure 1).
Moreover, grade three left varicocele was noted. Laboratory studies
including tumor markers- lactate dehydrogenase, beta human chorionic
progesterone and alpha-fetoprotein levels- as well as complete blood
count and kidney function tests were all within normal ranges.
MRI was recommended
for further characterization of the mass. This showed large complex
cystic lesion replacing large areas of the central left testis
8.5 cm. The lesion showed hypointense signal on T1 weighted images
and hyperintense signal on T2 weighted images (Figure
2) with a part of high signal on both T1 and T2 images at its superolateral
aspect in keeping with a hemorrhagic component. Post contrast images
also showed heterogeneous enhancement of the remaining testicular
tissue at its peripheral
inferior part (Figure 3).
Next day, additional staging CT scan showed no evidence of extra-testicular
metastasis, and the patient was consented for left radical orchiectomy.
Due to large size of the mass, simple trans-inguinal orchiectomy was not possible.
Instead, he underwent trans-inguinal and trans-scrotal orchiectomy
Histopatholgic examination showed sex cord stromal tumor of mixed forms composed
of three cell types; leydig, sertoli, and granulosa cells. Furthermore,
tumor margins, spermatic cord, tunica, and scrotal skin were free.
Immunohistochemistry was positive for Vimetin, calretinin, and
inhibin. Due to negative tumor
margins and absence of extra-testicular metastasis, no further
management was advised. At 24 months follow up; the patient was
doing well; an abdomen
and pelvis CT scan was normal.
Testicular cancer is relatively rare, accounting about 1-1.5% of all cancers
in men [2,5] however, it is the commonest
malignancy in the 15-34 years-old age group .
The majority of testicular cancers are germ cell tumors [2,5],
while sex cord stromal tumors are rare, representing 4%-6% of all testicular
neoplasms [1,6]. The prevalence of sex
cord tumors tends to be higher in pediatric patients with a frequency of 10-30%
Sex cord stromal tumors include; leydig, sertoli, granulosa, and theca cell tumors.
Also included are tumors that have mixed cell types (which may present as a mixture
of any combination of the mentioned cell types) and undifferentiated tumors .
Amongst these, leydig cell tumor is the most common histologic type [1,6].
The remaining histologic types are much less common .
Mixed sex cord tumor containing all three cell elements; granulosa-sertoli-leydig
cell testicular tumor is very rare with only three published cases in literature
The commonest presentation of testicular tumors is a painless scrotal mass.
In some cases, testicular neoplasm may initially be misdiagnosed as orchitis
Although 90% of non germ cell testicular tumors are benign, unfortunately, no
radiologic criteria allow differentiation of benign from malignant disease. Consequently,
orchiectomy is performed in all cases .
Ultrasonography is the primary imaging modality for investigating testicular
lesions. The primary goal is the mass localization (intratesticular or extratesticular),
and further lesion characterization (cystic or solid) .
Magnetic resonance imaging (MRI) may provide additional information in cases
where the Ultrasonography results are inconclusive [2,7]
and often affecting patient management .
To our knowledge, no descriptions of the MRI appearances of a mixed sertoli-ledyig-granulosa
cell tumor have been reported. On the other hand, radiological appearances of
leydig sex cord tumor and sertoli sex cord tumors were previously described [2,7].
In our case the imaging findings were those of a complex multilocular cystic
mass with necrotic and hemorrhagic components. However, larger study sample is
needed to characterize the radiological findings of mixed sex cord stromal tumors.
When it comes to tumor markers, Inhibin alpha is the most useful immunohistochemical
stain for identifying tumours of the sex cord stromal category. Expression of
calretinin and vimentin are also common which is concordant with our results
The optimal treatment of sex cord tumors is debatable. This is due to the infrequency
of these tumors, and the low percentage of malignant lesions .
The standard treatment method is inguinal orchiectomy. Testis sparing surgery
can be considered in certain conditions. There is no evidence to support additional
therapy in patients with disease clinically confined to testicle. Follow up should
be individualized according to patient status and the tumor aggressiveness properties.
Given the lack of extra testicular invasion, and the negative metastatic work
up, it was reasonable to treat this patient with orchiectomy alone. The patient
was followed for two years with out recurrence of the disease.
Although mixed sex cord tumors of the testis are rare in post pubertal
men, they should be considered in the differential diagnosis of
testicular masses in adults. The diagnosis can be challenging,
as it can present
with a long
history of testicular mass reaching large sizes. This case reflects
the clinical and radiological features of mixed sex cord tumor.
It further illustrates
the difficulty of differentiating it clinically and radiologically
from other lesions. Also it highlights the tumor potential for
proportions. Given the rarity of these tumors, and its challenging
imaging features, more data collection will be helpful in the management
The authors declare no competing interest.
Sakher Tahaineh, Rawan Abu Mughli, Moayid Fallatah. Study concept and design: Sakher Tahaineh. Acquisition of data: Sakher Tahaineh, Rawan Abu Mughli, Moayid Fallatah. Analysis and interpretation of data: Sakher Tahaineh, Rawan Abu Mughli, Moayid Fallatah. Drafting and editing of the manuscript: Rawan Abu Mughli. Critical review: Sakher Tahaineh, Moayid Fallatah. All author have approved the final manuscript for submission.
Figure 1: scrotal ultrasound shows mixed solid and multilocular cystic mass, containing cysts of varying echogenicties
Figure 2: sagittal T2- weighted MRI of the scrotum shows large intratesticular multilocular complex cystic lesion. Some of the cysts showed fluid fluid levels. The remaining testicular tissue appears of heterogeneous hyperintense signal with mild surrounding hydrocele
Figure 3: coronal T1 post contrast images shows heterogenous enhancement of the remaining peripherally displaces testicular tissue. And a cyst that showed hyper intense signal on both T1 pre contrast and T2, in keeping with a hemorrhagic cyst(star) at the superolateral aspect of the mass
Figure 4: gross specimen of the testicular tumor after removal from the patient
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