Outcomes and linkage to chronic care of HIV exposed infants among health centers and hospitals in Amhara Region, Ethiopia: implications to prevention of mother-to-child transmission of HIV program: a cross sectional study
Zemene Tigabu Kebede1,&, Belaynew Wasie Taye2
1Department of Pediatrics and Child Health, University of Gondar, Ethiopia, 2Departments of Epidemiology and Biostatistics, Bahir Dar University, Ethiopia
Zemene Tigabu, Department of Pediatrics and Child Health, University of Gondar, Ethiopia
numerous challenges exist in provision of prevention of mother-to-child transmission of HIV (PMTCT) such as linking HIV exposed infants (HEI) and their mothers to chronic cares services, and tackling loss to follow up. Limited evidence exists in Ethiopian setting that explains the persisting high HIV infection rate among HEIs and extent of linkage to chronic care. The study assessed the proportion of HIV infection;
children linked to chronic care and determinants of HIV infection among HEI in Northern Ethiopia.
this institution-based cross-sectional study was conducted in health centers
and hospitals of Amhara Region. A total of 484 HEI-mother pairs selected
by multistage random sampling were included in the study. Data were collected
from PMTCT and anti-retroviral therapy (ART) clinics using pre-tested
and structured questionnaires. Quantitative data were entered in Epi Info
version 7.0 and
exported to SPSS
20.0 for analysis.
a total of 484 mother-infant pairs with a response rate of 92.4% were included
in the analysis. About 94.2% of infants and women were linked to chronic
care follow-up sometime after the diagnosis. The proportion of HIV infection
12.4%. Antenatal care attendance had a significant association with HIV
infection among HEI (p<0.0001). Delivering in health institution (p<0.005),
mode of delivery (p<0.032), and provision of both infant (p<0.0001) and maternal
prophylaxis showed a highly significant association with HIV infection among
HIV exposed infants.
health facilities shall encourage antenatal care that increased institutional
delivery, leads to timely initiation and high uptake of PMTCT to reduce
the vertical transmission of HIV infection and meet national targets.
In Ethiopia, HIV prevalence among women aged 15-49 years is 1.9 percent
and increases with age to a peak of 3.7 percent at age 30-34 years.
Overall, HIV prevalence is higher in women than men in most age
More than 90% of the children who acquired HIV infection in 2011
live in sub-Saharan Africa. There, the number of children newly
infected fall by 24% from 2009
to 2011 . In 2011, the world made additional progress
in advancing towards the 2015 goal, generating significant confidence
in the feasibility of eliminating new infections
among children by 2015 . HIV infected
women seen in the pre-treatment era had 27% transmission rates, falling to only
1.9% in infants of women on highly active anti-retroviral therapy (HAART). Mortality
rate after HAART introduction is significantly lower than the period before the
availability of such therapy . Significant reduction in
mother-to-child transmission (MTCT) of HIV has been achieved with the introduction
of PMTCT in many countries of the world .
For example in South Africa  study revealed an infection
rate of 2.7% was observed and in Nigeria [6, 7]
it has fallen from 22.5% without PMTCT to 9.6 % with mother having received PMTCT.
HIV prevalence among HEI in Addis was 6.8% at age of six weeks .
Other studies found in the range of 5%  to 9.6% with PMTCT
and 10.5% without PMTCT [6, 9]. Studies
revealed that 64% took zidovudine (AZT) while 33% got ART and 73% of infants
ingested medication at birth .
The key factors that impact HIV infection among HIV Exposed Infants (HEI) are
maternal prophylaxis of either combination ART or single dose nevirapine (sdNVP);
mode of delivery, maternal age, and by feeding method [5
In order to correctly inform parents/caregivers of infant infection status and link HIV-infected infants to care and treatment, retention of both mothers and their infants is both crucial and challenging to the health system. Substantial implementation barriers, as well as personnel and infrastructure requirements, exist at each step in the cascade. Even with the highest reported levels of uptake, nearly half of HIV-infected infants may not complete the cascade successfully [7
South African study mortality rate was 1.7% (95% CI: 0.6% to .3%) and there were
94 (36.2%) lost to follow ups (LTFUs) by six months [5
Among 2477 HIV-exposed children registered for care by the United States Agency
for International Development-Academic Model Providing Access To Healthcare partnership
PMTCT program, 31 of 2477 infants (1.3%) were dead and 183 (7.4%) were lost to
follow-up by 3 months [9
]. Mother-infant pair enrolment in
the same facility, early antenatal care (ANC) attendance and the infant?s father
being tested and knew their HIV result were major predictors of infants adhering
to treatment and follow up [5
The study aimed to determine the proportion of HIV infection, identify associated
factors with HIV infection and linkage of HEI to chronic care in Amhara Region.
Study design: a cross-sectional study employing primary and secondary data was used among HEI in Amhara Region, Northern Ethiopia.
the study was conducted in selected health centers and hospitals in Amhara Regional State. The referral hospitals included Felegehiwot, Gondar, Debre Markos, and Dessie. The health centers are also situated in the same sites as the hospitals and are Gondar poly clinic, Debre Markos Health center, and Dessie health centers.
all mother-infant pairs in Amhara region were the source population. Mother-infant pairs in Amhara region residing in catchment areas of the selected health center/hospitals were the study population. Infants and young children aged 2 years and below; mothers or care takers of infants and young children residing in the selected areas; mothers and HIV exposed infants who had been tested for HIV and knew their status; infant?mother/caretaker pairs who had at least two follow-up data were included. Children referred for chronic care and/or further therapy to other institution and second and third born infants from multiple births were excluded from the study.
The dependent variables of the study were HIV infection, linkage to chronic care while explanatory variables of the study included: socio-demographic variables of mother/ caretaker (age, sex, residence, educational status, relation with child, monthly income, and number of pregnancies), and infant/child: (age, sex, presence of parents, and length of follow up), clinical care characteristics(gestational age at PMTCT start, place of delivery, mode of delivery, vaccination status of child, prophylaxis for child, any illness during pregnancy, gestational age at first visit, maternal sepsis, preterm labor, maternal mortality).Other variables are stage of HIV disease in mother, duration of HIV infection, CD4+ count of mother, viral load of mother, parity, mode of delivery, infant birth weight, sex of infant and feeding modality.
HIV Exposed Infant
was defined as an infant born to a mother with confirmed
HIV infection. Linkage to chronic care
was regarded as an infant born
to HIV infected mother or a mother who gave birth to HEI that is
to chronic care clinic for further follow up.
Sample size calculation and sampling techniques
Sample size was calculated using formula for single population proportions with the following assumptions: Z=1.96, standard normal distribution at 95%confidence level, p=6.8% (proportion of HEI who are HIV infected at six months using DNA/PCR) [8
], d=3% (margin of error), and Design effect=2; and 10% non-response rate. The final sample size was 524.
A multistage random sampling was employed to select HEI - mother pairs. At the first stage of sampling, a simple random sampling technique was applied using lottery method to identify health centers and hospitals. The second stage of sampling was selection of infant-mother pairs using a systematic random sampling technique from selected health centers and hospitals among infants and mothers coming for chronic care follow up using the daily attendance list in the clinic. This was implemented guaranteeing the proportional infant mother pairs in each facility as per the client flow.
Data collection procedures
A pre-tested, structured questionnaire developed in English and translated to Amharic that contained socio-demographic characteristics, family conditions, HIV status, and clinical care characteristics was used to collect quantitative data on infants and mothers. English version data extraction formats were used to retrieve some data from the charts of infants/children and their mothers. All the questionnaires and formats were pretested and corrected before the study resumed.
Data were collected by nurses and health professionals working outside of the facility under study who were given two days training. Daily supervision during the data collection period has been undertaken to maintain the quality of data and filled questionnaires were checked for completeness and accuracy.
Data quality control
To maintain the quality of data, the questionnaire was pre-tested and structured. Data collectors were adequately trained on interview techniques and measurements. Standard measuring tools for weight and height measurements were used. Completed questionnaires were checked daily. Supervision by the investigators throughout the data collection was carried out. Data were entered in to Epi Info software version 7.0 to control for error during data entry.
Data were entered, and cleaned using Epi Info version 7 software for windows and analyzed using SPSS 20 for windows. Descriptive statistics were used to present the socio-demographic characteristics, pregnancy related conditions, and magnitude of HIV infection. A chi-square test was used to identify determinants of HIV infection among HEIs. A p-value less than 0.05 was considered a statistically significant association.
Ethical approval was obtained from Institutional Review Board of the University of Gondar. Letter of permission was obtained from the School of Medicine, University of Gondar. Permission to pursue study was obtained from each facility. Informed consent was obtained from each mother/ guardian before the start of interview. The questionnaires were anonymous and no names of children or mothers were used. The data collected from each participant were kept confidential and locked. Any infant or mother had the right to withdraw at any point during data collection.
Socio-demographic characteristics of women
A total of 484 mother-infant pairs were studied giving a response rate of (92.4%). Nearly 20 questionnaires were excluded due to significant missing information. Most (91.7%) women were from urban areas. More than half (56.2%) of them were in the age range of 20-29 years. Three-forth (75.2%) of mothers were married and two hundred eighty five (58.9%) were house wives while nearly half (46.9%) of women achieved educational level of secondary or above. One-third (33.7%) of women have delivered 5 or more times (Table 1
Characteristics of infants
A total of 484 children (50.2% females and 49.8% males) were included in the study. Majority (80.8%) of children were 6-12months old with the mean (+s.d.) age of 10.9 (+ 9) months. The mean birth-weight of infants was 2.9 (+ 0.6) kilograms. More than two-thirds of infants lie in the range of 2.5-4.0 kilograms (Table 2
Pregnancy and delivery characteristics
Majority (94.4%) of women had antenatal care follow up for the current pregnancy and the same proportion (94.0%) of women delivered in health institutions. Most (94.4%) said that they had no problems during pregnancy while the rest reported to have some form of illness or pregnancy related problem. Spontaneous vertex delivery (SVD) constituted for most of the modes of deliveries where they had delivered without episiotomy (81.6%) or with episiotomy (11.6%). About 6.8% delivered through cesarean section (C/S). Among all, 8.5% had no any prophylaxis during pregnancy or delivery (Table 3
Linkage to chronic care of infants and children
About 94.2% of infants and women were linked to chronic care follow-up sometime after the diagnosis while 5.8% were not linked during the required time and the same 94.2% were given appointment after delivery for arrangement of further follow up. However, the appointment dates were not suitable for 16.9% of women who have been given appointments for follow-up. The reach of care for the mother was either very easy or easy for 55.4% and 41.7% of women respectively, while it was difficult or very difficult for 2.5% and 0.4% of women respectively. Fifty six (11.6%) of women declared that they were not sent to other clinics in the same facility when they were required to attend (Table 4
Infant HIV infection and correlates
Among 484 infants served in all the facilities studied, the level of HIV infection was 12.4%. There is no difference between males and females with regards to HIV infection (P=0.81). At the same time, residence (rural or urban) has no association with HIV infection (p=0.3). Antenatal care attendance has a significant impact on the occurrence of HIV infection among HEI (p<0.0001). Delivering in health institution also resulted in lesser HIV infections (p<0.005) as is having no problems during delivery (p<0.028) and mode of delivery (p<0.032). Provision of both infant (p<0.0001) and maternal (p<0.0001) prophylaxis have a highly significant association with prevention of HIV infection among HIV exposed infants (Table 5
This research identified the level of HIV infection among HEI and the extent to which linkage to chronic care has been implemented in the region. A sample of health center and hospitals were included in the study.
The level of HIV infection (12.4%) among HEI in Amhara region has been still high despite a major intervention by the Ministry of Health with a national logo of ?no child shall be born HIV infected?. A study in Addis Ababa reported a 6.8% infection rate; is lower than this study. One of the reasons is the study in Addis Ababa included the result from DNA/PCR at six months while this study included all until the age of 18 months that might have increased the rate as infections after six months of age are detected . This is much higher than studies in other settings  where 5% were HIV infected. This might be partly due to the high burden of HIV in this country. The other study had reported 7.4% losses-to-follow up and 1.3% deaths which were not included in the analysis as HIV status were not known. Those LTFUP might have been infants with HIV infection thereby underestimating the HIV prevalence.
However, this was lower than findings from Nigeria [6
] where the overall average infection rate was 22.5% which might be due to inclusion of many mothers with no PMTCT as compared to our study where the PMTCT coverage was high. Similar higher rates were also reported from other studies [4
In this study antenatal care follow-up has a positive impact in preventing transmission of HIV among HEI (P<0.001).This is because antenatal care is an opportunity to get maternal prophylaxis or ARVs that significantly reduces vertical transmission. Other studies in Zambia showed a significant reduction of vertical transmission of HIV [11
The place of delivery is also found to be significantly associated with HIV
infection in HEI again due to an increased opportunity for babies delivered
in health facilities to get infant prophylaxis thereby protection from infection. The
mode of delivery has also impact on infection as some operative deliveries like
episiotomy increase the risk of infection and the C/S reduces duration of labor
and hence lesser risk of infection.
The decreased risk of HIV infection among HEI in those who were on either infant or maternal prophylaxis shows the health system need to reach all women for PMTCT and encourage institutional delivery accompanied by proper provision of follow up and treatment.
The level of HIV infection among HEI in Amhara region remains high despite all interventions. Maternal antenatal care, institutional delivery, cesarean delivery, and provision of infant and maternal prophylaxis were positive predictors of preventing HIV infection among HEI. A significant portion of infants and mothers identified as HIV exposed or infected respectively were left unlinked to the chronic HIV care. This leads to missed opportunity to prevent HIV infection in exposed infants and to provide treatment in HIV infected women.
The health facilities shall encourage antenatal care that entails increased institutional delivery, high uptake of PMTCT and infant prophylaxis to reduce the vertical transmission of HIV infection and meet national targets. Health facilities shall reorganize the care system from labor ward to the chronic care unit to effectively reach each woman to continue HIV care and provide testing and subsequent therapy of exposed children.
What is known about this topic
- Prevalence of HIV infection in exposed infants before the scale up of PMTCT;
- The key factors that impact HIV infection among HIV Exposed Infants (HEI); maternal prophylaxis; mode of delivery, maternal age, and feeding method.
What this study adds
- Prevalence of HIV infection among exposed infants after PMTVT scale up;
- Linkage to chronic care of HIV infected infants;
- Place of delivery, antenatal care and complication during delivery as correlates of HIV infection in exposed infants.
The Authors declare no competing interests.
Zemene Tigabu wrote the proposal, involved in the data collection,
analysis and interpretation. Belaynew Wassie was involved in data collection,
analysis and interpretation of statistical outputs and drafted the manuscript.
Both authors read and approved the final manuscript.
The investigators would like to forward their thanks
to the University of Gondar for providing the time to conduct the study.
Our gratitude also goes to the Ethiopian Network for HIV/AIDS Treatment
Care and Support (USAID ENHAT-CS) program, which is funded by president's
emergency plan for AIDS Relief (PEPFAR) and implemented by an Management
Science for Health (MSH) led consortium of international and Ethiopian
organizations, for supporting this research. <b>Disclaimer:</b> this
publication was made possible by the generous support of the United States
Agency for International Development (USAID) under Request For Application
(RFA): 663-11-000005. The contents are the responsibility of the authors
and do not necessarily reflect the views of USAID or the United States
Table 1: demographic characteristics of HIV positive mothers in Amhara region, May-July 2013
Table 2: basic characteristics of HIV exposed infants in Amhara region, Northwest Ethiopia, May-July 2013
Table 3: pregnancy, delivery and maternal prophylaxis among HIV infected women in Northwest Ethiopia, May-July 2013
Table 4: chronic care linkage of infants and women, in Northwest Ethiopia, May-July 2013
Table 5: determinants of HIV infection among HEI in Amhara region, Northwest Ethiopia, May -July 2013
- Central Statistical Agency [Ethiopia] and ICF International. Ethiopia Demographic and Health Survey 201 Addis Ababa, Ethiopia and Calverton, Maryland, USA: 2012. Google Scholar
- Joint United Nations Programme on HIV/AIDS (UNAIDS). UNAIDS Report on the global AIDS epidemic. UNAIDS, 2012. Google Scholar
- Noel F, Mehta S, Zhu Y et al. Improving outcomes in infants of HIV-infected women in a developing country setting. PLoS one. 2008;3(11):e3723. PubMed | Google Scholar
- Harjot K, Nikhil G, Aarti K et al. High rates of all-cause and gastroenteritis related hospitalization morbidity and mortality among HIV-exposed Indian infants. BMC Infectious Diseases. 2011;11:193. PubMed | Google
- Terusha C, Stephen K, Janet G, Tamaryn L, Lisa M, Marie-Louise N. A retrospective study of Human Immunodeficiency Virus transmission, mortality and loss to follow-up among infants in the first 18 months of life in a prevention of mother-to-child transmission programin an urban hospital in KwaZulu-Natal, South Africa. BMC Pediatrics. 2012; 12(146). Google Scholar
- Abayomi J, Niyi A, Abieyuwa E et al. Outcome of PMTCT
services and factors affecting vertical transmission of HIV infection in Lagos,
HIV and AIDS Review. 2011; 10(1):14-18. Google
- Andrea L, Ji-Eun P, Lynn R, Kenneth A, Rochelle P, Valeriane L. Early infant HIV-1 diagnosis programs in resource limited settings: opportunities for improved outcomes and more cost-effective interventions. BMC Medicine. 2011; 9(59). Google Scholar
- Mulatu B, Frida E, Addisalem A. Prophylactic treatment uptake and compliance with recommended follow up among HIV exposed infants: a retrospective study in Addis Ababa, Ethiopia. BMC Research Notes. 2011; 4:563. Google Scholar
- Nyandiko W, Otieno-Nyunya B, Musick B, et al. Outcomes
of HIV-exposed children in western Kenya: efficacy of prevention of mother
to child transmission in a resource-constrained setting. J Acquir Immune Defic
Syndr. 2010; 54(1): p. 42-50. PubMed | Google
- Alemnesh H, Sven G, Mitike M, Karen M and Odd M. Current status of medication adherence and infant follow up in the prevention of mother to child HIV transmission programme in Addis Ababa: a cohort study. Journal of the International AIDS Society. 2011; 14(50). PubMed | Google Scholar
- Torpey K, Mandala J, Kasonde P et al. Analysis of HIV early infant diagnosis data to estimate rates of perinatal HIV transmission in Zambia. PLoS One. 2012; 7(8): e42859. PubMed | Google Scholar