Table 1: list of PCR primers and aplicon sizes of target genes

Primers

Nucleotide sequence(5’-3’)

Amplicon size (bp)

References

ctxB (Fw-con)

ACTATCTTCAGCATATGCACATGG

947

Morita et al. (2008)

ctxB (Rv-classical)

CCTGGTACTTCTACTTGAAACG

 

ctxB (Rv-El-Tor)

CCTGGTACTTCTACTTGAAACA

 

tcpA, El Tor-Fw

GAAGAAGTTTGTAAAAGAAGAACAC

472

Keasler et al. (1993)

tcpA, El Tor-Rv

GAAGGAACCTTCTTTCACGTTG

 

tcpA, classical-Fw

CACGATAAGAAAACCGGTCAAGAG

618

Keasler et al. (1993)

tcpA, classical-Rv

ACCAAATGCAACGCCGAATGGAGC

 

rtxC-Fw

CGACGAAGATCATTGACGAC

265

Chow et al. (2001)

rtxC-Rv

CATCGTCGTTATGTGGTCGC

 

CtxA-Fw

CTCAGACGGGATTTGTTAGGCACG

302

Keasler et al. (1993)

ctxA-Rv

TCTATCTCTGTAGCCCCTATTACG

 

ctxB-F

GGTTGCTTCTCATCATCGAACCAC

400

Olsvik et al. (1993)

Ctxb-R

GATACACATAATAGAATTAAGGAT