Table 1: list of PCR primers and aplicon sizes of target genes |
|||
Primers |
Nucleotide sequence(5’-3’) |
Amplicon size (bp) |
References |
ctxB (Fw-con) |
ACTATCTTCAGCATATGCACATGG |
947 |
Morita et al. (2008) |
ctxB (Rv-classical) |
CCTGGTACTTCTACTTGAAACG |
|
|
ctxB (Rv-El-Tor) |
CCTGGTACTTCTACTTGAAACA |
|
|
tcpA, El Tor-Fw |
GAAGAAGTTTGTAAAAGAAGAACAC |
472 |
Keasler et al. (1993) |
tcpA, El Tor-Rv |
GAAGGAACCTTCTTTCACGTTG |
|
|
tcpA, classical-Fw |
CACGATAAGAAAACCGGTCAAGAG |
618 |
Keasler et al. (1993) |
tcpA, classical-Rv |
ACCAAATGCAACGCCGAATGGAGC |
|
|
rtxC-Fw |
CGACGAAGATCATTGACGAC |
265 |
Chow et al. (2001) |
rtxC-Rv |
CATCGTCGTTATGTGGTCGC |
|
|
CtxA-Fw |
CTCAGACGGGATTTGTTAGGCACG |
302 |
Keasler et al. (1993) |
ctxA-Rv |
TCTATCTCTGTAGCCCCTATTACG |
|
|
ctxB-F |
GGTTGCTTCTCATCATCGAACCAC |
400 |
Olsvik et al. (1993) |
Ctxb-R |
GATACACATAATAGAATTAAGGAT |